Restriction Enzyme - Action of EcoRI
The action of restriction enzymes
Restriction enzymes, also called restriction nucleases (EcoRI in this
example) , surrounds the DNA molecule at the point it seeks(sequence
GAATTC). It cuts one strand of the DNA double helix at one point and
the second strand at a different, complementary point (between the G and
the A base). The separated pieces have single stranded "sticky-ends,"
which allow the complementary pieces to combine.
The newly joined pieces are stabilized by DNA ligase.
EcoRI, one of many restriction enzymes, is obtained from the bacteria